Sailgene Technology
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service@sailgene.comIntroduction
Spatial transcriptome sequencing enables comprehensive mapping of gene expression within the intact tissue architecture.We provide integrated Visium HD and Stereo-seq spatial transcriptomics services. Our dual-platform solution delivers comprehensive spatial insights, combining the high-definition, subcellular resolution of Visium HD with the expansive field of view and ultra-high sensitivity of Stereo-seq. This powerful approach enables detailed mapping of tissue architecture and gene expression for breakthrough discoveries in biology and disease research.
Applications
Growth and Development Research
Organ Studies
Evolutionary Research
Disease Research
Highlights
spatial gene expression information
subcellular resolution
high sample compatibility
Workflow
Sample QC
Library QC
Data QC
Bioinformatics Analysis
Refer to the demo results
Analysis Workflow
Data Delivery File Formats
Fastq Data Files
Sequencing Yield Display
Mouse embryo:
Human ovarian cancer :
Sample Requirements
| Sample Type | Platform | Sample Collection | Transportation |
| OTC | Visium HD 3' |
Ensure that fresh tissues are embedded in OCT within 30 minutes after ex vivo isolation. Length × Width: ≤ 6.5 mm × 6.5 mm; Height: ≥ 5 mm Mark the cut surface with an asterisk (*) or the label "Cut Surface". Stored at -80℃. |
Pack the sorted samples and embed them in dry ice and send it out as soon as possible. Ensure sufficient dry ice is used. |
| Stereo-seq |
Ensure that fresh tissues are embedded in OCT within 30 minutes after ex vivo isolation. The maximum size of the tissue cut surface shall not exceed 0.9 times the chip size. The thickness of the embedded block shall be controlled within 2 cm. Mark the cut surface with an asterisk (*) or the label "Cut Surface". Stored at -80℃. |
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| Spatial Full length cDNA | Visium HD 3' or Stereo-seq | Quantity Requirements: ≥ 50ng/flow cell Criteria:The fragment size of cDNA is approximately ≥500 bp. Stored at -80°C. |
Pack the sorted samples and embed them in dry ice and send it out as soon as possible. Ensure sufficient dry ice is used. |
Publications
| Year | Journal |
Impact Factor |
Title |
| 2026 | Nature Plants | 13.6 | Host microenvironment in potato-Phytophthora infestans interaction revealed by single-cell spatiotemporal transcriptome. |
| 2025 | Nature Genetics | 29.0 | High-definition spatial transcriptomic profiling of immune cell populations in colorectal cancer. |
| 2025 | Immunity | 26.3 | Recirculating regulatory T cells mediate thymic regeneration through amphiregulin following damage. |
| 2025 | Nature Cancer | 28.5 | Spatially resolved transcriptomics and graph-based deep learning improve accuracy of routine CNS tumor diagnostics. |
| 2025 | Nature Communications | 15.7 | Spatiotemporal transcriptome and metabolome landscapes of cotton somatic embryos. |
What's the best choice?
| 10x Visium | 10x Visium CytAssist | 10x Visium HD | Stereo-seq | |||
| Sample Preparation | Fresh Frozen | Fresh Frozen /FFPE | Fresh Frozen/FFPE | Fresh Frozen /FFPE | ||
| Chip Size | 6.5*6.5mm | 6.5*6.5m/11*11mm | 6.5*6.5mm | 5*5mm/10*10mm | ||
| Capture Area | 4 | 2 | 2 | 1 | ||
| Spot Size and Count |
55um, about 5000 spots |
55μm, about5000/14000 spots |
2μmx2μm(squares), about 11 million spots |
0.22μm,100/400 million spots |
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| Resolution | 100μm | 100μm | 2μm | 0.5μm | ||
| Quality Standard | RIN>7 | RIN>4 | DV200>30% | DV200>50% | DV200>30% | RIN>7/DV200>30% |
| Section Thickness | 10μm | 5μm | 5μm | 10μm | ||
| Tissue Optimization | ✓ | ✕ | ✕ | ✓ | ||
| Slide | Manual | Automatic | Automatic | Manual | ||
| Principle | PolyA capture | Probe hybridization | Probe hybridization |
PolyA capture/Random primers |
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| Applicable Species | All | only human and mice | only human and mice | All | ||
|
Measurement Sequence |
90G raw data |
60G raw data(6.5*6.5mm) 120G raw data(11*11mm) |
120G raw data |
500M raw reads(5*5mm) 1G raw reads(10*10mm) |
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| Release Year | 2019 | 2022 | 2024 | 2021 | ||
Keyword:
Spatial Transcriptomics
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA
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