Sailgene Technology
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service@sailgene.comIntroduction
Directly sequence full‑length transcripts from 5’ UTR to poly‑A tail.
Iso‑seq is based on PacBio SMRT sequencing. It captures full‑length cDNA without fragmentation or assembly, enabling accurate analysis of alternative splicing, fusion genes, alternative polyadenylation (APA), and novel transcripts. It also improves quantification resolution from gene level to isoform level.
Highlights
High precision: Base accuracy ≥Q30, reliable for SNP and splicing variant detection.
Complete structure: Full‑length sequences for AS, APA, fusion gene detection.
Better annotation: Refine reference genomes and build high‑quality transcript sets for non‑reference species.
Product Advantages
Genome annotation improvement – Refine gene sets, discover novel genes/transcripts.
Transcript structure analysis – AS, APA, fusion genes, lncRNA, CDS, SSR.
Growth & development regulation – Transcript dynamics across stages/tissues.
Metabolism & resistance – Key pathway regulation for molecular breeding.
Disease mechanism – Differential transcripts & functional enrichment.
Why choose Iso‑seq?
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Capture full‑length transcripts – No assembly required, no information loss.
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Resolve isoform complexity – Identify alternative splicing events and isoform‑level expression.
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Ideal for non‑model organisms– Works without a reference genome.
Recommended sequencing strategies
|
Application Goal |
Recommended HiFi Reads (per sample) |
Biological Replicates |
|
Transcript structure only |
2.5 – 5 Mb |
Not required |
|
Quant. + structure |
5 – 10 Mb |
≥3 |
|
Genome annotation (genome size ≤1 Gb) |
≤1 G:5 Mb; 1–3 G:10 Mb; ≥3 G:>10 Mb |
Multitissue RNA pooled |
|
Genome annotation (genome size >1 Gb) |
≥10 Mb |
Multitissue pool |
Keyword:
Iso-Seq Full-Length Transcriptome
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA
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