HiFi Bacterial Complete Genome-PacBio HiFi Sequencing-Sailgene Technology

Sailgene Technology

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HiFi Bacterial Complete Genome

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Introduction
Why HiFi for bacteria?
Technical Parameters

Introduction

Complete, gap‑free bacterial genomes – from a single run.

PacBio HiFi sequencing delivers highly accurate long reads (≥Q30, 10–25 kb), enabling complete assembly of bacterial genomes – including chromosomes and plasmid sequences. No need for short‑read polishing or gap‑closing steps.

Why HiFi for bacteria?

Complete assemblies – Resolve repetitive regions, rRNA operons, and insertion sequences.

High accuracy – Base accuracy >99.9% (Q30) eliminates extra polishing.

Plasmid resolution – Capture both circular and linear plasmids in the same assembly.

Methylation detection – Simultaneous 5mC and 6mA detection without additional library preparation.

Technical Parameters

Sequencing Platform	Sequencing Strategy	Assembly Metrics	Sample Submission Requirements
Revio	100X	1 contig, 0 gaps (no guarantee for plasmids)	DNA concentration ≥50 ng/µL; total DNA ≥8 µg

Keyword:

HiFi Bacterial Complete Genome

Iso-Seq Full-Length Transcriptome

HiFi Fungal Genome

If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

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WhatsApp：1-(617)-223-7544

Tel：1-(617)-223-7544

E-mail：service@sailgene.com

Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA