Name: Direct RNA Sequencing
Brand: Transcriptome
Availability: InStock

Sailgene Technology

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Direct RNA Sequencing

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Introduction
Applications
Highlights
Library Construction Workflow
Analysis Workflow
Data Delivery File Formats
Sequencing Yield Display
Sample Requirements
Publications
What's the best choice?

Introduction

Direct RNA Sequencing: Without reverse transcription or amplification, it enables direct reading of full-length poly(A)-tailed RNA molecules (rather than cDNA). This technology can detect RNA modification sites (e.g., m6A, m5C, pseudouridine ψ, inosine, and 2'-O-Me) on individual RNA molecules, enabling precise analysis of alternative splicing, fusion genes, and the identification of novel isoforms. Additionally, it provides relatively accurate estimation of poly(A) tail length, thereby preserving the native RNA characteristics.

Applications

Developmental Biology Research

Oncology Research

Immunoinfection Research

Environmental Toxicology Research

Highlights

Detect RNA modifications (m5C, m6A, ψ, inosine, 2'-O-Me, etc.) at single-nucleotide resolution.

Accurately estimate poly(A) tail length.

Avoid the introduction of false-positive transcripts via direct RNA sequencing without reverse transcription or amplification.

Library Construction Workflow

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Analysis Workflow

Direct RNA Sequencing.png

Data Delivery File Formats

FASTQ and POD5 Data Files

Sequencing Yield Display

Species	TotalBase	TotalReads	N50	meanQ
Human	24,186,756,830	21,156,455	1,685	21.49
Human	26,236,107,976	20,699,814	1,781	21.79
Plant	16,221,740,860	18,232,141	958	21.59
Animal	24,079,670,614	24,346,060	1,213	21.56
Animal	25,781,032,891	24,731,182	1,404	22
Animal	20,875,860,663	17,684,923	1,681	22
Animal	19,551,477,538	20,416,360	1,296	21.65
Animal	20,325,290,079	18,077,586	1,589	21.71
Cell	22,865,483,808	17,417,836	1,938	20.43
Mammal	20,875,860,663	17,684,923	1,681	22
Mammal	20,325,290,079	18,077,586	1,589	21.71
Mammal	22,710,639,651	23,941,576	1,296	21.65
Plant	19,398,123,548	21,691,091	1,209	21.84
Plant	18,097,091,216	20,688,982	1,161	21.34

Sample Requirements

Sample Types	Amount	RNA Integrity	Purity
Total RNA	Data>10Gb: ≥15 μg; Data<10Gb: ≥6 μg;	The RNA sample is clear and transparent, with no insoluble matter. The 28S and 18S main bands are clear, with 28S/18S ratio ≥ 1, and the insect 18S band is clear without any trailing.	A260/280=1.9-2.1; A260/230≥1.0

Tissue Type	Direct RNA Sequencing
Dry Weight of Fresh Animal Tissue	≥1 g
Dry Weight of Fresh Plant Tissue	≥2 g
Freshly Cultured Cells	≥1-5×10⁷
Freshly Collected Whole Blood	10-15 ml
Microbial Biomass	≥5×10⁷ or ≥0.2 g

Publications

Year	Journal	Impact Factor	Title
2025	Nature Communications	15.7	A mutually antagonistic mechanism mediated by RNA m6A modification in plant-virus interactions
2025	Science Bulletin	21.1	Epi-transcriptome analysis reveals the lack of N6-methyladenosine modification in two RNA viruses infecting watermelon
2025	Genome Biology	10.1	Widespread impact of transposable elements on the evolution of post-transcriptional regulation in the cotton genus Gossypium
2025	Molecular Cell	14.5	Single-molecule m6A detection empowered by endogenous labeling unveils complexities across RNA isoforms
2025	Developmental Cell	10.7	Nanopore RNA direct sequencing identifies that m6A modification is essential for sorbitol-controlled resistance to Alternaria alternata in apple
2024	Signal Transduction and Targeted Therapy	40.8	Characterization of ACTN4 as a novel antiviral target against SARS-CoV-2

What's the best choice?

	Full-length transcript sequencing	Tail Iso-seq	Direct RNA-seq	Direct cDNA-seq	Full-Length lncRNA Sequencing	Long non-coding RNAs Sequencing	PacBio iso-seq	RNA-seq
Gene quantification	+++	+++	+++	+++	+++	+++	+	+++
Gene difference analysis	+++	+++	+++	+++	+++	+++	+	+++
Isoform quantification	+++	+++	+++	+++	+++	+	+	+
Isoform difference analysis	+++	+++	+++	+++	+++	+	+	+
Alternative splicing	+++	+++	+++	+++	+++	+	+++	+
fusion gene	+++	+++	+++	+++	+++	+	+++	+
novel transcript prediction	+++	+++	+++	+++	+++	+	+++	+
Allele analysis	+++	+++	+++	+++	+++	NO	+++	+
Alternative polyadenylation	+++	+++	+++	+++	+++	+	+++	+
Poly(A) length	NO	+++	+++	NO	NO	NO	NO	NO
LncRNA analysis	+	+	+	+	+++	+++	NO	+
Modification analysis (m6A, m5C, Ψ, I)	NO	NO	+++	NO	NO	NO	NO	NO
Positive and negative chain analysis	+++	+++	+++	+++	+++	+++	+	NO
Library Construction	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Without reverse transcription and PCR amplification	Only reverse transcription	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification	Reverse transcription and PCR amplification
Sequencing platform	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Nanopore PromethION	Illumina	PacBio Revio	Illumina

Keyword:

Direct RNA Sequencing

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Full-length Transcriptome Sequencing

If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

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