Telomere-to-Telomere (T2T) Genome

Introduction


The Telomere-to-Telomere (T2T) genome represents a complete and gapless assembly of anorganism's genome, spanning from one telomere to the other. Unlike conventional assembliesthe T2T genome leverages advanced long-read sequencing technologies-PacBio HiFi and Nanopore ultra-long reads-in combination with Hi-C scaffolding to produce highly contiguousaccurate, and comprehensive genomic assemblies. As a gold standard in genome assemblythe T2T genome provides a powerful foundation for investigating genome architecture,evolutionary biology, functional genomics, and unlocking new frontiers in genomic research.

Applications


Segmental duplication and variation research

Research on genes located near centromere

Functional gene identification & genetic variation analysis

Epigenetic research

Centromere diversity research

Highlights


Independently developed Nanopore ultra-long DNA extraction kits and successfully sequenced over 200 species using Nanopore ultra-long read technology.

Developed and patented a proprietary telomere-to-telomere (T2T) genome assembly method.

Published 20+ high-impact T2T genome studies in leading joumals over the past two years.

Workflow


Sample Preparation

Sample Preparation

Refer to: Sample Submission Guidelines

Extraction

Extraction

Refer to Ultra-long DNA Extraction Protocol / DNA Extraction Protocol

Sample QC

Extraction
Library Preparation

Library Preparation

Refer to Ultra-long DNA Library Construction Workflow / PacBio Revio DNA Library Construction Workflow

Library QC

Library Preparation
Sequencing

Sequencing

Platform: -Short-read Sequencing: Illumina/DNBSEQ; -Long-read Sequencing: Nanopore PromethION / PacBio Revio

Data QC

Sequencing
Bioinformatics Analysis

Bioinformatics Analysis

Pan-genome Demo Report

Click >>

Analysis Workflow


Telomere-to-Telomere (T2T) Genome.png

Publications


Year

Journal

Impact Factor

Title

2025

nature genetics

31.8

A telomere-to-telomere genome assembly coupled with multi-omic data provides  insights into the evolution of hexaploid bread wheat

2025

Molecular Plant

24.1

Two complete telomere-to-telomere Medicago genomes reveal the landscape and evolution of centromeres

2025

Nature Communications

16.6

Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species

2025

Plant Communications

11.6

A telomere-to-telomere reference genome assembly of tomato cultivar Heinz 1706

2025

Plant Biotechnology Journal

12.1

A telomere-to-telomere gap-free assembly integrating multi-omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranate

Contact Us

If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

Contact Us
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E-mail:service@sailgene.com

WhatsApp:1-(617)-223-7544

Tel:16172237544

Email:service@sailgene.com

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