Nanopore Ultra-long Sequencing (PromethION platform)

Introduction


Nanopore platforms enable single-molecule sequencing by directly detecting changes in electrical current as DNA strands pass through nanopores. This technology supports read lengths of hundreds of kilobases (kb). When combined with Sailgene’s proprietary ultra-long DNA extraction kit, read lengths with an N50 exceeding 150 kb can be achieved.

SAILGENE TECHNOLOGY INC.

Applications


Whole genome sequencing

T2T genome assembly

Identification of genetic structure variations

Improved continuity of assembly

Human genetic diseases research

Highlights


Sequencing produces ultra-long reads directly, eliminating the need for fragment screening

Sequencing reads N50 up to 100-200 kb

Samples from various eukaryotic organisms are accepted

Addressing the sequencing challenges of complex regions

Workflow


Sample Preparation

Sample Preparation

Refer to: Sample Submission Guidelines

Extraction

Extraction

Refer to Ultra-long DNA Extraction Protocol

Sample QC

Extraction
Library Preparation

Library Preparation

Refer to Ultra-long DNA Library Construction Workflow

Library QC

Library Preparation
Sequencing

Sequencing

Nanopore PromethION

Data QC

Sequencing
Bioinformatics Ananlysis

Bioinformatics Ananlysis

Demo Report

Click >>

Library Construction Workflow


SAILGENE TECHNOLOGY INC.

Data Delivery File Formats


Fastq and POD5 Data Files

Data Delivery Standard


 

Sample Types

Data yield per cell (Gb) (N50 > 150K)

Conventional species

Plants

rice, maize, wheat,

soybean, peanut, etc.: >15G

Conventional forest trees: >10G

Animals

Mammals, including humans, primates, pigs, cattle, etc.: fresh blood >15G
Freshwater fish, mammals (sheep): >10G

Sequencing Yield Display


Kingdom

Family/Category

Data yield per cell (Gb)(N50>150K)

Q

Plants

Ericaceae

20

24

Salicaceae

17

23

Rosaceae

22

24

Brassicaceae

20

24

Moraceae

25

24

Fabaceae

15

24

Solanaceae

16

24

Poaceae

16

24

Amaranthaceae

16

24

Animals

Mammal

20

24

Freshwater fish

15

24

Inset

10

24

 

Sample Requirements


1.Plant samples are recommended to send newly grown young leaves.

 

2.Animal samples are recommended to give preference to fresh blood, followed by liver and other tissues.

 

3.Unconventional samples do not guarantee sequencing yield.

 

Nanopore sequencing unconventional samples are as follows:

 

1) plants with a lot of secondary metabolites;

 

2) oceans and aquatic products (animals and plants, algae, etc.);

 

3) amphibians, birds, etc.

Publications


Year Journal Impact Factor Title
2025 nature genetics 31.8 A telomere-to-telomere genome assembly coupled with multi-omic data provides  insights into the evolution of hexaploid bread wheat
2025 Molecular Plant 24.1 Two complete telomere-to-telomere Medicago genomes reveal the landscape and evolution of centromeres
2025 Nature Communications 16.6

Comparative genomics provides insights into the biogeographic and biochemical diversity of meliaceous species

2025 Plant Communications 11.6 A telomere-to-telomere reference genome assembly of tomato cultivar Heinz 1706
2025 Plant Biotechnology Journal 12.1

A telomere-to-telomere gap-free assembly integrating multi-omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranate


Keyword:

Nanopore Ultra-long Sequencing (PromethION platform)


Contact Us

If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.

Contact Us
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Contact Us

E-mail:service@sailgene.com

WhatsApp:1-(617)-223-7544

Tel:16172237544

Email:service@sailgene.com

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