Sailgene Technology
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service@sailgene.comIntroduction
10X Genomics single-cell RNA sequencing is a high-throughput cell capture technology based on microfluidics, droplet encapsulation, and barcode labeling. It enables the simultaneous isolation and labeling of 500–10,000 single cells, capturing the 3' or 5' transcriptome information of each cell. This approach allows for the study of gene expression profiles at high throughput and single-cell resolution, revealing the heterogeneity of complex cell populations and avoiding the masking of individual cell gene expression signals by population averaging.
Applications
Organs/Tissue Cell Atlas
Developmental Biology Research
Disease-Related Cell Type Discovery
Cancer Research
Virology Research
Highlights
Rare Cell Subtype Identification
True Single-cell Resolution
High Throughput
Multi-Omics Integration
Precision Medicine Potential
Workflow
Suspension QC
Library QC
Bioinformatics Analysis
Refer to the demo report
Analysis Workflow
Data Delivery File Formats
Fastq Data Files
Sequencing Yield Display
Plant leaf:
Mouse embryo:
Sample Requirements
| Technical Type | Sample Type | Sample Collection | Transportation |
| Single-cell RNA Sequencing | Animal |
Quantity Requirements: Snap-freeze in liquid nitrogen for 20–30 minutes, then store at -80°C or ship immediately. |
Pack the sorted samples and embed them in dry ice and send it out as soon as possible.Ensure sufficient dry ice is used. |
| Plants | Quantity Requirements: 1–2 g Criteria: Prioritize tender, well-growing, and representative plants. Snap-freeze in liquid nitrogen for 30 minutes, then store at -80°C or ship immediately. |
Publications
| Year | Journal |
Impact Factor |
Title |
| 2025 | Nature Neuroscience | 20 | GABA-dependent microglial elimination of inhibitory synapses underlies neuronal hyperexcitability in epilepsy |
| 2025 | Nature Cancer | 28.5 | Skeletal muscle endothelial dysfunction through the activin A–PGC1α axis drives progression of cancer cachexia |
| 2025 | Nature Immunology | 27.6 | Bat organoids reveal antiviral responses at epithelial surfaces |
| 2025 | Immunity | 26.3 | Maternal natural killer cells drive neuroimmune disorders in offspring through aberrant secretion of extracellular granzyme B |
| 2025 | Cell | 42.5 | Post-gastrulation amnioids as a stem cell-derived model of human extra-embryonic development |
What's the best choice?
|
Long-read Single-Cell Transcriptome Sequencing |
Single-cell transcriptome sequencing |
|
| Sequencing Technology | Nanopore PromethION | Illumina |
| Reads Length | Reads N50:~1kb | 150bp |
| Delivery File Format | Fastq | Fastq |
| alyze Content |
1、Gene expression,cell clustering,cell annotation 2、Identification of isoform diversity 3、Isoform quantification 4、Differential transcript analysis for cell clustering 5、Comparison of gene clustering results with isoform clustering results 6、Identification of cell subpopulations through differential alternative splicing 7、Isoform switching between cell populations 8、Identification of fusion genes in cell clusters 9、Identification of IncRNAs in cell clusters |
1、Gene expression, cell clustering,cell annotation |
Keyword:
Single-Cell Transcriptome Sequencing
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA
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