Sailgene Technology
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service@sailgene.comIntroduction
SMART Bacterial Complete Genome refers to first assembling the genome using high-accuracy next-generation sequencing (NGS) data (Illumina or BGI platforms). During the assembly process, Nanopore long reads are used to bridge branch points and connect them into a complete genome map, followed by final error correction using NGS data. This strategy facilitates the rapid generation of high-quality bacterial complete genome maps, lowering the error rate to 0.001%.
Applications
Pathogenic Microorganisms
Industrial Fields: Antibiotic production, yogurt fermentation, energy utilization, etc.
Environmental Science
Product Advantages
Faster Turnaround: Complete all analytical work within one week after sequencing completion.
More Cost-Effective: Achieve more research outcomes with lower funding input.
Higher Accuracy: Accuracy is guaranteed by next-generation sequencing (NGS) data, with an error rate as low as 0.001%. This is one order of magnitude lower than the error rate of direct assembly using third-generation sequencing data followed by error correction.
No Bias: Free from GC bias, enabling uniform coverage across the entire genome. It is the preferred choice for both high-GC and low-GC genomes.
Workflow
Data QC
Fast And Comprehensive Bioinformatics Analysis
Library Preparation Flowchart
Nanopore Sequencing Experimental Workflow
Analysis Flowchart
Data Delivery Format
FASTQ
Comparison with Other Technologies
|
Dimension |
SMART Bacterial Complete Genome Map |
Next-Generation Bacterial Genome |
|
Outcome |
High-quality complete genome (complete map) |
Scaffold map |
|
Assembly |
1 Contig, 0 Gaps, error rate 0.001% |
Multiple Contigs with Gaps, low error rate but incomplete |
|
Long Reads |
Next-generation short reads + ONT long reads |
Short reads only (200-500 bp) |
|
Coverage of Complex Regions |
Uniform coverage of high-GC/repetitive regions |
Large coverage bias in extreme regions |
|
Library Preparation |
No PCR required, single-molecule sequencing |
Relies on PCR amplification |
|
Structural Variation (SV) Detection |
Accurate identification of all types of SVs |
Difficult to detect large-fragment SVs |
|
Epigenetic Modification Detection |
Direct detection, no additional experiments required |
Additional experiments required |
Article Case
1. Development of “environmentally friendly” super Escherichia coli strains that can completely biodegrade toluene
Publication Date: January 15, 2025
Journal & Impact Factor: Chemical Engineering Journal; 13.3
DOI: 10.1016/j.cej.2025.159877
Citation: Wang Y, Qian C, Deng Y-D, et al. Development of "environmentally friendly" super Escherichia coli strains that can completely biodegrade toluene. Chem Eng J. 2025; 506:159877.
2.: Carbon dioxide enhances Akkermansia muciniphila fitness and anti-obesity efficacy in high-fat diet mice
Publication Date: February 23, 2025
Journal & Impact Factor: The ISME Journal; 10.8
DOI: 10.1093/ismejo/wraf034
Citation: Wang X, Yang Q, Shi C, Wang Y, Guo D, Wan X, Dong P, Zhang Q, Hu Y, Zhang R, Yang H, Chen W, Liu Z. Carbon dioxide enhances Akkermansia muciniphila fitness and anti-obesity efficacy in high-fat diet mice. ISME J. 2025 Jan 2;19(1):wraf034.
Sample Submission Requirements
Microbial Whole Genome Sequencing Specifications:
DNA Sample Requirements
|
|
Sample Type |
Amount |
Volume |
Concentration |
Purity (NanoDropTM/Agarose Gel) |
|
Microbial whole genome library (Illumina/BGI) |
Genomic DNA |
≥200 ng |
≥20 μL |
≥10 ng/μL |
OD260/280=1.8-2.0 no degradation no contamination |
|
Nanopore PromethION DNA library |
High Molecular Weight Genomic DNA
|
≥2 μg |
≥20 μL |
≥100 ng/μL |
OD260/280=1.8-2.0 OD260/230≥1.8 NC/QC=0.95-1.2 no degradation no contamination |
Library TypeOther types of samples can be consulted with the application scientist
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA
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