Sailgene Technology
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service@sailgene.comIntroduction
DNA methylation plays a crucial role in maintaining normal cellular functions, genetic imprinting, embryonic development, and the onset of human tumors, making it one of the hottest areas of research today. Nanopore sequencing eliminates the need for bisulfite conversion or immunoprecipitation enrichment experiments, enabling direct detection of multiple DNA modifications, including 5mC, 5hmC,6mA, and 4mC.
Applications
Whole Genome Methylation Profiling
DNA Methylation in Disease Research
Epigenetic Regulation of Gene Expression
Methylation-Based Biomarker Discovery
DNA Methylation in Population Studies
Highlights
Comprehensive modification detection: Detect multiple base modifications, such as 5mC,5hmC,6mA, and 4mC in a single experiment, offering high cost-efficiency.
Long read sequencing: Enables the identification of methylation sites in complex regions like repeats or transposons.
No GC bias: Achieves more balanced genome coverage and detects more methylation sites.
Dual insights from a single dataset: Obtain both structural variation (SV) and DNA methylation data simultaneously.
Library Construction Workflow
Analysis Workflow
Data Delivery File Formats
Deliver both FastQ format data and POD5 format data.
Data Delivery Standard
Sample Requirements
|
Sample Type Platform |
Plant tissue sample |
Animal tissue sample |
Number of cells cultured |
Blood |
Bacterial sample |
fungi |
|
Nanopore DNA methylation |
≥500 mg |
≥500 mg |
≥1*107 |
≥3 mL |
≥500 mg |
≥500 mg |
Publications
| Title | Journal | Impact Factor | Year |
| The correlation between CpG methylation and gene expression is driven by sequence variants | Nature Genetics | 31.7 | 2024 |
| DNA methylation govems the sensitivity of repeats to restriction by the HUSH-MORC2 corepressor | Nature Communications | 14.7 | 2024 |
| Long-read sequencing of diagnosis and post-therapy medulloblastoma reveals complex rearrangement patterns and epigenetic signatures | Cell Genomics | 11.1 | 2023 |
| Locus-resolution analysis of L1 regulation and retrotransposition potential in mouse embryonic development | Genome Research | 6.2 | 2023 |
What's the best choice?
|
Feature |
WGBS |
Nanopore DNA Methylation |
|
Sequencing Platform |
Illumina |
Nanopore |
|
Read Length |
PE150 |
No length limitation, enabling ultra-long reads for comprehensive analysis of complex genomic regions |
|
Bisulfite Treatment Required |
Yes |
No |
|
Detectable Methylation Types |
5mC |
5mC, 5hmC (5-hydroxymethylcytosine), 6mA (6-methyladenine),4mC (4-methylcytosine); allows for direct detection without chemical modification |
|
Data Formats Delivered |
Fastq |
POD5, Fastq |
|
Key Advantages |
High accuracy with short reads |
No length limitation、Direct detection、Versatile applications、Enhanced resolution |
Keyword:
Nanopore DNA Methylation
Contact Us
If you are interested in our long-read sequencing services or potential collaboration, please contact us. Our team is ready to support your research with tailored solutions. We also welcome feedback from users to help us improve our services.
Contact Us
Add: One Innovation Drive, Suite B3-406, Worcester, MA 01605, USA
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